Effect of 17beta-estradiol on phosphorylcholine cytidylyltransferase activity from cultured rat lung explants / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 57-59, 2003.
Article
em Zh
| WPRIM
| ID: wpr-339680
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>AIM</b>To investigate the influence and mechanisms of 17beta-estradiol on the CTP: phosphorylcholine cytidylyltransferase (CCT) activity from cultured lung explants without serum.</p><p><b>METHODS</b>We detected the amount of [M-14C] choline incorporation into phosphatidylcholine so as to reflect CCT activity by liquid scintillation.</p><p><b>RESULTS</b>(1) 17beta-estradiol increased the CCT activity in dose-dependence and time-dependence. (2) Both the protein kinase C inhibitor H-7 and calmodulin antagonist W-7 abolished the stimulatory effect of 17beta-estradiol (3 x 10(-6) mol/L) on the CCT activity.</p><p><b>CONCLUSION</b>17beta-estradiol can increase CCT activity in cultured lung explants, its mechanism is related to protein kinase C and calmodulin.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Farmacologia
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Técnicas In Vitro
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Proteína Quinase C
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Calmodulina
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Meios de Cultura Livres de Soro
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Ratos Wistar
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Colina-Fosfato Citidililtransferase
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Estradiol
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Pulmão
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Metabolismo
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2003
Tipo de documento:
Article