The establishment of a new resequencing pathogen microarray-based assay for detecting unexplained respiratory tract infections / 病毒学报
Chinese Journal of Virology
; (6): 137-142, 2013.
Article
em Zh
| WPRIM
| ID: wpr-339962
Biblioteca responsável:
WPRO
ABSTRACT
Resequencing Pathogen Microarray (RPM) is a new pathogen detection and identification technology based on DNA microarray. In order to apply RPM in the detection of unexplained infection and as a result, to improve the emergency response capacity, a new RPM-based respiratory pathogens detection assay was developed to simultaneously detect 19 common respiratory viruses, 9 influenza A viruses (Flu A),11 human rhinoviruses(HRV), 28 enteroviruses and 18 rare respiratory viruses. The specificity of multiplex system was examined by confirmed positive specimens for 16 common respiratory virus. The sensi-tivity was evaluated by serial ten-fold dilutions of plasmids or in vitro-transcribed RNA. RPM could detect and differentiate 16 virus types/subtypes at 10 - 1 000 copies/reaction level. Nucleic acids of 8 throat swabs with unexplained respiratory tract infections were pooled and detected by the new assay. The RPM result was verified by common PCR followed by sequencing as well as PLEX-ID (Abbott). Except for a false-positive of PIV1, no difference among the three assays was found. These results indicate the assay based on the new RPM is a highly sensitive, high throughput test for the detection of respiratory virus infections, which is significant for the management of emergent and epidemic infectious disease.
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Índice:
WPRIM
Assunto principal:
Infecções Respiratórias
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Virologia
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Vírus
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Sensibilidade e Especificidade
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Classificação
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Análise de Sequência com Séries de Oligonucleotídeos
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Diagnóstico
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Genética
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Métodos
Tipo de estudo:
Diagnostic_studies
Limite:
Child
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Child, preschool
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Female
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Humans
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Male
Idioma:
Zh
Revista:
Chinese Journal of Virology
Ano de publicação:
2013
Tipo de documento:
Article