Establishment and identification of A new HPV positive esophageal cancer cell line / 病毒学报
Chinese Journal of Virology
; (6): 119-125, 2013.
Article
em Zh
| WPRIM
| ID: wpr-339965
Biblioteca responsável:
WPRO
ABSTRACT
In order to explore the relationship between human papilloma virus ( HPV) and upper gastrointestinal cancer(esophageal cancer), An esophageal squamous cell carcinoma(ESCC) tissue was obtained from a 76 year old Chinese female patient from Anyang city, a high-incidence area for esophageal cancer, in China. Transplanted tumor was formed through direct SCID mouse tumorigenicity experiment and cultured monolayer cells were obtained after several passages and screenings Immunofluorescence test, cell growth curve, soft agar assay, chromosome analysis and tissues HE staining were also performed to confirm the epithelial cell origin. Cell DNA STR typing results showed that no three alleles was observed,indicating no contamination of human cells. DNA analysis revealed the presence of HPV type 18 DNA in this cell line. DOLINK test found the E6 protein expression of HPV virus. We concluded that the established cell line is a new esophageal squamous cell-origincarcinoma cell line with HPV DNA positive and expression of viral oncoprotein. It provides new cytologic material for performing etiology studies on the occurrence and development of esophageal cancer.
Texto completo:
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Índice:
WPRIM
Assunto principal:
Infecções Tumorais por Vírus
/
Virologia
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Neoplasias Esofágicas
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Células Tumorais Cultivadas
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Carcinoma de Células Escamosas
/
China
/
Camundongos SCID
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Infecções por Papillomavirus
/
Biologia Celular
/
Proliferação de Células
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
/
Female
/
Humans
/
Male
País/Região como assunto:
Asia
Idioma:
Zh
Revista:
Chinese Journal of Virology
Ano de publicação:
2013
Tipo de documento:
Article