The influence on the growth of endothelial cells onto titanium-oxide films modified by poly-L-lysine and immobilized with fibronectin / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 864-869, 2008.
Article
em Zh
| WPRIM
| ID: wpr-342725
Biblioteca responsável:
WPRO
ABSTRACT
In this work, a surface biological and chemical modification method was used for improving the biological behavior of endothelial cells onto titanium-oxide films. The titanium-oxide films were first activated by HCl and H2O2 to produce hydroxyl group, then coated with poly-L-lysine and further immobilized with fibronectin. The surface characteristics of samples were analyzed by Fourier Transfer Infrared Spectrum, X-ray Photoelectron Spectroscopy and contact angle method. The biological behavior of cultured human umbilical vein endothelial cell (HUVEC) seeding onto different samples surface was evaluated by the in vitro HUVEC original cultured experiment. The results showed that the method of coating with poly-L-lysine and immobilizing with fibronectin can promote the adhesion and growth of endothelial cells onto titanium-oxide film.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Farmacologia
/
Polilisina
/
Titânio
/
Veias Umbilicais
/
Adesão Celular
/
Células Cultivadas
/
Química
/
Fibronectinas
/
Células Imobilizadas
/
Materiais Revestidos Biocompatíveis
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2008
Tipo de documento:
Article