The study of apoptosis and mechanism of cells exposed to steep pulse / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 637-641, 2008.
Article
em Zh
| WPRIM
| ID: wpr-342774
Biblioteca responsável:
WPRO
ABSTRACT
This experiment was designed to study the apoptosis and related mechanism of adherent liver tumor cells (SMMC-7721) and adherent normal liver cells (HL-7702) when they were exposed to the steep pulse generated by the steep pulse apparatus for tumor treatment. The results showed that the steep pulse of 200 V could induce tumor cells apoptosis. The tumor cells presented with their apoptosis when they were exposed to the steep pulse from 200 V to 250 V. Laser scanning confocal microscopy was used to make a real time study of calcium burst when the adherent tumor cells were exposed to the steep pulse. The results showed:On the condition of no extracellular Ca2+, the concentration of Ca2+ in tumor cells exposed to the steep pulse of 150 V did not change; the concentration of Ca2+ in tumor cells exposed to the steep pulse of 200 V decreased; the concentration of Ca2+ in tumor cells exposed to the steep pulse of 250 V decreased more evidently. On the condition of existing extracellular Ca2+, the concentration of Ca2+ in tumor cells exposed to the steep pulse of 150 V did not change; the concentration of Ca2+ in tumor cells exposed to the steep pulse of 200 V decreased little; the concentration of Ca2+ in tumor cells exposed to the steep pulse of 250 V reduced little, too. Maybe the change of calcium burst in the tumor cells is the mechanism of apoptosis when cells are exposed to the steep pulse.
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Índice:
WPRIM
Assunto principal:
Patologia
/
Efeitos da Radiação
/
Células Tumorais Cultivadas
/
Cálcio
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Apoptose
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Microscopia Confocal
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Hepatócitos
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Biologia Celular
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Eletricidade
/
Campos Eletromagnéticos
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2008
Tipo de documento:
Article