Preparation and identification of monoclonal antibody against PNAS-2 protein / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1269-1272, 2009.
Article
em Zh
| WPRIM
| ID: wpr-343304
Biblioteca responsável:
WPRO
ABSTRACT
This study was purposed to prepare and primarily identify the specific monoclonal antibodies (McAbs) against the apoptosis related protein PNAS-2 so as to provide the essential tool for study of PNAS-2 function. The McAbs against PNAS-2 were prepared via the immunization of mice, cell fusion and cloning using synthetic peptide of PNAS-2 as immunogen; the specificity, titer and subtype of McAb were detected by Western blot, ELISA and immunofluorescence. The results showed that the stable hybridoma cell line S-31-7 producing McAbs against PNAS-2 protein was successfully obtained. The immunoglobulin of the McAb was identified to be IGg1lambda. The titer of ascetic fluid fled McAb were 1:8,000. A single specific band with 28 kD was shown in Western blot test, and the antigen recognized was present in cell cytoplasm by immunofluorescence. In conclusion, the obtained McAb against PNAS-2 displays strong specificity and high titer, which may be applied to the advanced research on PNAS-2 protein.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Alergia e Imunologia
/
Proteínas Reguladoras de Apoptose
/
Camundongos Endogâmicos BALB C
/
Anticorpos Monoclonais
/
Especificidade de Anticorpos
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Ano de publicação:
2009
Tipo de documento:
Article