Influence of ethylbenzene on oxidative damage and apoptosis in rat renal epithelial cells NRK-52e / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
; (12): 133-136, 2013.
Article
em Zh
| WPRIM
| ID: wpr-343673
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the oxidative damage and apoptosis of renal tubular epithelial cells (NRK-52e cell line) induced by ethylbenzene.</p><p><b>METHODS</b>NRK-52e cells were exposed to 30, 60, 90, 120 μmol/L ethylbenzene for 24 hours. Cell viability were measured using MTT, the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT), the contents of malondialdehyde (MDA) and glutathione (GSH) were detected respectively. PI fluorescent staining assay was applied to detect percentage of apoptosis in ethylbenzene-treated groups.</p><p><b>RESULTS</b>Compared with control group, cell outline became clear, cell diopter increased, cell became smaller and shrinkage, some cells broke in 60 μmol/L ethylbenzene-treated group. Plenty of cells died, suspension cells increased significantly in 90 μmol/L ethylbenzene-treated group. Compared with control group, cell viability the activities of SOD and CAT and the content of GSH were significantly decreased in 60 and 90 μmol/L ethylbenzene-treated groups (P<0.05). The MDA content were remarkably elevated in 90 μmol/L ethylbenzene-treated groups (P<0.05).</p><p><b>CONCLUSION</b>Ethylbenzene can induce oxidative stress and apoptosis in NRK-52e cells (P<0.05).</p>
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Índice:
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Assunto principal:
Oxirredução
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Derivados de Benzeno
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Linhagem Celular
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Espécies Reativas de Oxigênio
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Apoptose
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Estresse Oxidativo
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Biologia Celular
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Células Epiteliais
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Toxicidade
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Túbulos Renais
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Ano de publicação:
2013
Tipo de documento:
Article