Influence of Qingdai compound on expression of bcr/abl and JWA in K562 cells / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 809-811, 2005.
Article
em Zh
| WPRIM
| ID: wpr-343882
Biblioteca responsável:
WPRO
ABSTRACT
To study the effects of Qingdai compound on proliferation and apoptosis of K562 cells, as well as the expression of bcr/abl and JWA mRNA, K562 cells were treated in culture with different concentrations of Qingdai compound (2.5, 5, 7.5, 10 and 20 mg/ml) and harvested at 24 hours. Then morphological changes were observed by light microscopy (LM); expressions of bcr/abl and JWA were detected with semi-quantitative RT-PCR. The results showed that morphological changes were observed as the increment of the Qingdai compound concentration. Inhibition effects on proliferation and apoptosis in K562 cells were seen. A concentration-dependent decreases were found in bcr-abl and JWA mRNA expression of K562 cells. Qingdai compound partially inhibited proliferation and induced apoptosis of K562 cells. Expressions of both bcr/abl and JWA, which took part in cell proliferation and apoptosis, were down-regulated in a dose dependent manner. In conclusion, Qingdai compound can partially inhibit the expressions of bcr/abl and JWA genes in K562 cells, and the clinical effect of Qingdai compound on CML may be associated with apoptosis of leukemic cells.
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Índice:
WPRIM
Assunto principal:
Farmacologia
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RNA Mensageiro
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Medicamentos de Ervas Chinesas
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Regulação Neoplásica da Expressão Gênica
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Proteínas de Fusão bcr-abl
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Apoptose
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Células K562
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Reação em Cadeia da Polimerase Via Transcriptase Reversa
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Peptídeos e Proteínas de Sinalização Intracelular
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Proliferação de Células
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Ano de publicação:
2005
Tipo de documento:
Article