A pilot study on establishment of human/pig hematopoietic chimera model in fetal and neonatal pigs / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 673-676, 2005.
Article
em Zh
| WPRIM
| ID: wpr-343907
Biblioteca responsável:
WPRO
ABSTRACT
This study was aimed to explore the feasibility of transplanting human cord blood stem cells (HSC) into pre-immune fetal and neonatal pigs, and to investigate the self-renewal of HSC in the recipient pigs. The fetus and neonate were manipulated in sterile separated room and human donor cells were injected into fetus via fetus muscle or umbilical vein (dissectted womb) or into neonate via umbilical vein before cutting it. Human CD45(+) cells s were detected by labeling with human anti-CD45 antibody and analyzed by fluorescence activated cell sorting (FACS). The results showed that tested pigs developed as well as control and a definite proportion of human cells existed in peripheral blood of chimeric pig on day 60 after transplantation. In conclusion, the fetus and neonate pigs can tolerate a definite proportion of human antigens, and to establish the human/pig model of hematopoietic chimerism is possible.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Suínos
/
Transplante Heterólogo
/
Sangue
/
Projetos Piloto
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Antígenos Comuns de Leucócito
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Quimeras de Transplante
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Modelos Animais
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Transplante de Células-Tronco de Sangue do Cordão Umbilical
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Alergia e Imunologia
/
Feto
Limite:
Animals
/
Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Ano de publicação:
2005
Tipo de documento:
Article