Effect of matrine on human ether à go-go related gene (HERG) channels expressed in Chinese hamster ovary cells / 中国结合医学杂志
Chinese journal of integrative medicine
; (12): 430-434, 2010.
Article
em En
| WPRIM
| ID: wpr-344923
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of matrine on human ether à go-go related gene (HERG) potassium channels expressed in Chinese hamster ovary (CHO) cells and investigate whether HERG channel is a new target of the pharmacological effect of matrine on arrhythmia and tumor</p><p><b>METHODS</b>HERG channel potassium current in CHO cell was recorded using whole-cell patch-clamp technique, and the influence of matrine on the current was explored.</p><p><b>RESULTS</b>Matrine inhibited HERG potassium current in a dose-dependent manner, and the 50% inhibitory concentration (IC IC(50)) was 411±23 μmol/L. Matrine had no significant effect on the activation kinetics, and mainly blocked HERG channels in their closed state.</p><p><b>CONCLUSIONS</b>The blocking effect of matrine on HERG channels might be one of the mechanisms against arrythmias and tumors. Unlike most other blockers exerting blocking effect at the intracellular sites by entering the cell with the opening of HERG channel, matrine blocked HERG channels at the extracellular sites.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Farmacologia
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Quinolizinas
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Cricetulus
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Células CHO
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Alcaloides
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Canais de Potássio Éter-A-Go-Go
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Canal de Potássio ERG1
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Genética
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Metabolismo
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Chinese journal of integrative medicine
Ano de publicação:
2010
Tipo de documento:
Article