Influence on the cytokines expression on hepatic tissue by inhibition the signal pathway of inflammatory mediators following extensive hepatectomy in rats / 中华外科杂志
Chinese Journal of Surgery
; (12): 6-9, 2005.
Article
em Zh
| WPRIM
| ID: wpr-345042
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the impact of AG490, a cytokine signaling inhibitor, on cytokine signaling pathway with phosphorylation levels of Janus kinase 2 (Jak2) and singal transducers and activators of transcription 3 (Stat3), and liver pro-/anti-inflammatory cytokine expressions.</p><p><b>METHODS</b>Rats were divided into two groups after surgery: control group, without treatment; AG490 group, with AG490 (1 mg.kg(-1).12 h(-1)) administration intraperitoneally, immediately and through 36 hs after the operation. Western blotting was used to detect the levels of phosphorylated Jak2 and Stat3. Semi-quantitative RT-PCR was employed to examine Interleukin-6 (IL-6) and Interleukin-10 (IL-10) expression.</p><p><b>RESULTS</b>At 8 h and 12 h post-operatively, the phosphorylation levels of Jak2 and Stat3 were significantly inhibited in the AG490 group when compared with the control group. The DNA levels of IL-6 in the liver of the AG490 group rat at the same time points were also decreased, whereas IL-10 levels markedly increased. These changes made the ratio of IL-6/IL-10 dropped significantly.</p><p><b>CONCLUSIONS</b>AG490 ameliorates the overwhelming inflammatory response via a mechanism of blocking cytokine signaling transduction and consequently suppresses the ratio of pro-/anti-inflammatory cytokine expression, which exerts potential clinical implications of use of anti-inflammatory agents in hepatic surgery.</p>
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Índice:
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Assunto principal:
Farmacologia
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Fisiologia
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Proteínas Tirosina Quinases
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Transdução de Sinais
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Transativadores
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Citocinas
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Proteínas Proto-Oncogênicas
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Interleucina-6
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Interleucina-10
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Ratos Sprague-Dawley
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Surgery
Ano de publicação:
2005
Tipo de documento:
Article