Study on optimization of induction system of test-tube tuberous roots from leaves of Rehmannia glutinosa / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 3812-3814, 2012.
Article
em Zh
| WPRIM
| ID: wpr-346832
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WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of sucrose and plant growth substances of different concentrations on the induction of test-tube tuberous roots of Rehmannia glutinosa, in order to establish an efficient system for the induction of test-tube tuberous roots from leaves of R. glutinosa.</p><p><b>METHOD</b>Leaves from test-tube seedlings of 85-5 R. glutinosa were used as explants. After rooting induction, they were transferred to medium with orthogonal design for inducing test-tube tuberous roots of R. glutinosa.</p><p><b>RESULT AND CONCLUSION</b>NAA played a significant role in induction of test-tube tuberous roots of R. glutinosa, followed by sucrose and 6-BA. With leaves from test-tube seedlings as the explants, the optimal medium for inducing test-tube tuberous roots of R. glutinosa was MS + BA 3.0 mg x L(-1) + NAA 0.1 mg x L(-1) + sucrose 7%. The study provides an efficient induction system for studies on artificial seeds and secondary metabolism with test-tube tuberous roots of R. glutinosa.</p>
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Assunto principal:
Farmacologia
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Reguladores de Crescimento de Plantas
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Purinas
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Sacarose
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Compostos de Benzil
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Raízes de Plantas
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Folhas de Planta
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Rehmannia
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Plântula
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Técnicas de Cultura de Tecidos
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2012
Tipo de documento:
Article