Ectopic bone induction in vivo after transplantation of skeletal satellite cells from green fluorescence protein transgenic mouse transfected by adenoviral vectors encoding bone morphogenetic protein-2 / 华西口腔医学杂志
West China Journal of Stomatology
; (6): 504-507, 2007.
Article
em Zh
| WPRIM
| ID: wpr-348007
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the ability of induced ectopic bone using skeletal muscles satellite cells (SMSCs) from newborn green fluorescence protein (GFP) transgenic mice mediated by Ad-BMP2.</p><p><b>METHODS</b>Transplantation of SMSCs transduced with Ad-BMP2 into back lamb muscles of subfascia in wildtype 129sv mice with a complex of collagen scaffords, then the tissue histologic examination, X ray plain film, fluorescence microscopy were used.</p><p><b>RESULTS</b>Transplantation of SMSCs transfected with Ad-BMP2 into back lamb muscles of subfascia generated ectopic bone formation involving GFP-positive osteoblasts and osteocytes 2 weeks and mature bone formation 4 weeks after transplantation. SMSCs non-transfected with Ad-BMP2 failed to induce ectopic bone formation.</p><p><b>CONCLUSION</b>SMSCs retain differentiation potentitality into osteoblasts in response to Ad-BMP2. They are useful tools for analyzing the process of osteoblast differentiation in vivo after transplantation.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Osteoblastos
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Osso e Ossos
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Camundongos Transgênicos
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Transfecção
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Diferenciação Celular
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Fator de Crescimento Transformador beta
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Proteínas Morfogenéticas Ósseas
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Mioblastos
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Proteínas de Fluorescência Verde
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Proteína Morfogenética Óssea 2
Limite:
Animals
Idioma:
Zh
Revista:
West China Journal of Stomatology
Ano de publicação:
2007
Tipo de documento:
Article