Screening of cellular proteins binding to the core region of hepatitis C virus RNA by ultraviolet cross-linking assay / 中华肝脏病杂志

ABSTRACT

<p><b>OBJECTIVE</b>To screen cellular proteins binding to the core region of hepatitis C virus (HCV) from human hepatoma cells.</p><p><b>METHODS</b>Unlabeled and labeled RNA transcripts were prepared by in vitro transcription. Cytoplasmic extracts were prepared from human hepatoma cells HepG2. Ultraviolet (UV) cross-linking was used to screen the cellular proteins that would bind to the core region of HCV. Competition experiment was performed to confirm the specificity of the binding in which excess unlabeled RNA of HCV core region and plasmid RNA were used as competitors.</p><p><b>RESULTS</b>Two cellular proteins of 6.6 x 10(4) and 5.5 x 10(4) were found binding to the core region of HCV RNA by UV cross-linking assay. The unlabeled core region of HCV RNA could compete out this binding whereas the unlabeled plasmid RNA could not.</p><p><b>CONCLUSION</b>The cellular proteins from HepG2 cells could bind to the core region of HCV RNA.</p>