Quality standard study on Entadae Semen / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1860-1864, 2015.
Article
em Zh
| WPRIM
| ID: wpr-351249
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish the quality standard of Entadae Semen, and provide scientific basis for its quality control.</p><p><b>METHOD</b>TLC and HPLC were used for qualitative identification and quantitative analysis of phaseoloidin and entadamide A-O-β-D-glucopyranoside in Entadae Semen. The test of water content, ash and ethanol-soluble extractives of Entadae Semen was carried out according to the methods recorded in appendix of Chinese Pharmacopeia (2010 edition).</p><p><b>RESULT</b>The TLC was well separated with clear spots. The linear range of phaseoloidin was between 0.014 to 2.747 g x L(-1) (r = 0.999 6, n = 9) with an average recovery rate of 101.06% (RSD 0.90%, n = 6); the linear range of entadamide A-O-β-D-glucopyranoside was between 0.002 to 0.452 g x L(-1) (r = 0.999 7, n = 9) withan average recovery rate of 101.52% (RSD 1.09%, n = 6). The content of phaseoloidin in sample is between 5.12% to 9.24%, entadamide A-O-β-D-glucopyranoside is between 0.55% to 2.17%, alcohol-soluble extracts is between 30.9% to 45.2%, water is between 6.6% to 8.6%, and total ash is between 2.4% to 2.9%.</p><p><b>CONCLUSION</b>The established standard is acceptable for quality control of Entadae Semen.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Controle de Qualidade
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Padrões de Referência
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Medicamentos de Ervas Chinesas
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Química
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China
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Cromatografia Líquida de Alta Pressão
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Cromatografia em Camada Fina
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Fabaceae
Tipo de estudo:
Qualitative_research
País/Região como assunto:
Asia
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2015
Tipo de documento:
Article