Effect of sodium butyrate in combination with ATRA on the proliferation/differentiation of MDS cell line SKM-1 / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 601-605, 2004.
Article
em Zh
| WPRIM
| ID: wpr-352010
Biblioteca responsável:
WPRO
ABSTRACT
The study was purposed to explore the molecular mechanisms of sodium butyrate (NaB) action on SKM-1 cell proliferation/differentiation and to study its synergistic effect with all-trans retinoic acid (ATRA). SKM-1 cells were grown in the absence or presence of NaB and/or ATRA; the percentage of viable cells was determined by trypan blue exclusion; differentiation was investigated by nitro-blue tetrazolium (NBT) reduction; adhesion molecules of cell surface were analysed by FACS; cell cycle distribution was studied after DNA staining by propidium iodide; D-type cyclins, CDK and P21 mRNA were detected by reverse transcription-polymerase chain reaction (RT-PCR). The results showed that NaB and/or ATRA blocked cells mainly in the G0/G1 phase of the cell cycle; ATRA inhibited the mRNA expression of CDK6, CDK4, cyclin D3 and cyclin D1; NaB inhibited the mRNA expression of CDK2, cyclin D2 and cyclin D1; ATRA and NaB inhibited the mRNA expression of CDK6, CDK4, CDK2, cyclin D1, cyclin D2 and cyclin D3; ATRA and/or NaB both stimulated p21 expression at the mRNA levels. It is concluded that the NaB effect on cell proliferation/differentiation may be linked to its ability to induce expression of p21 mRNA and inhibit the cyclin D-CDK complexes. These observations support the claim that NaB has the synergistic effect with ATRA.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Patologia
/
Farmacologia
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Tretinoína
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Síndromes Mielodisplásicas
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Butiratos
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Ciclo Celular
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Diferenciação Celular
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Linhagem Celular
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Ciclinas
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Proteínas de Ciclo Celular
Limite:
Aged
/
Humans
/
Male
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Ano de publicação:
2004
Tipo de documento:
Article