Interferon-γ enhances human γδ T cell-mediated osteosarcoma cell killing in vitro / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the cytotoxic effect of γ&delta; T cells from osteosarcoma patients against interferon-γ (IFN-γ)-treated osteosarcoma cells in vitro.</p><p><b>METHODS</b>Human γ&delta; T cells were amplified by zoledronate from peripheral blood cells of osteosarcoma patients. The expression of Fas on the osteosarcoma cells were measured by flow cytometry and quantitative real-time PCR analysis before and after IFN-γ treatment. The cytotoxicity of γ&delta; T cells against osteosarcoma cells was evaluated with LDH assay.</p><p><b>RESULTS</b>IFN-γ significantly enhanced the susceptibility of the osteosarcoma cell lines HOS and U2OS to the cytotoxicity of γDelta; T cells from osteosarcoma patients (P<0.01). IFN-γ obviously up-regulated the expression of Fas in HOS and U2OS cells (P<0.01). Anti-FasL mAb failed to inhibit the cytotoxicity of γ&delta; T cells in untreated osteosarcoma targets (P>0.05), but significantly impaired γ&delta; T cell cytotoxicity in IFN-γ pre-treated osteosarcoma targets (P<0.01).</p><p><b>CONCLUSION</b>IFN-γ can enhance the cytotoxic effect of human γ&delta; T cells from osteosarcoma patients against osteosarcoma cells in vitro.</p>