Anti-Mur screening and Mur antigen genotyping of voluntary blood donors in Guangzhou / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the frequency of anti-Mur and Mur antigen among blood donors in Guangzhou to provide evidence for guiding clinical transfusion and prenatal screening.</p><p><b>METHODS</b>DG Gel Coombs cards were used to screen active anti-Mur at 37 degrees celsius; from 2725 blood donors. Multiplex ligation-dependent probe amplification (MLPA) was used to genotype Mur antigen from 91 blood donors, and human anti-Mur serum was used to verify the phenotypes deduced from the genotypes.</p><p><b>RESULTS</b>The frequency of anti-Mur and genotyped Mur antigen was 0.04% (1/2725) and 6.59% (6/91), respectively, and the phenotyping results were consistent with the genotyping results.</p><p><b>CONCLUSION</b>The blood donors in Guangzhou show a low frequency of anti-Mur and a relatively high frequency of Mur antigen. Genotyping using MLPA allows Mur antigen genotyping when commercial anti-Mur is not available.</p>