Interaction between polysaccharides and interferon-gamma using an improved ELISA approach / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
; (6): 315-325, 2004.
Article
em Zh
| WPRIM
| ID: wpr-353314
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish an ELISA approach to study the interaction of polysaccharides with cytokine in vitro.</p><p><b>METHODS</b>The heparin BSA complexes (HBC) were synthesized with a chemistry method and separated using a 1 X 90 cm column of Separose 4B. After identification of the complex via SDS-PAGE,the wells of ELISA plates were coated with HBC and the interaction of HBC with interferon-gamma (IFN-gamma) was detected. The effects of heparin, low molecular weight heparin (LMW heparin), chondroitin sulfate (CS), hyaluronic acid (HA) and carrageenans on the binding of HBC to IFN-gamma were tested in this system.</p><p><b>RESULT</b>Human recombinant IFN-gamma bound to heparin in a concentration dependent manner, the binding of IFN-gamma to HBC was detected at the concentration of 0.25 ng, and saturated at around 2 ng. Free heparin, LMW heparin, CS,HA and carrageenans competed for the binding of IFN-gamma to HBC with significant different ability. The IC(50)concentrations of heparin and LMW heparin were 2.40 microg/ml and 18.60 microg/ml respectively.</p><p><b>CONCLUSION</b>IFN-gamma is a cytokine with high binding affinity to heparin and carrageenans family but poor to CS-A and CS-C. ELISA is a simple, sensitive approach to detect the interaction of polysaccharides with cytokine in vitro.</p>
Texto completo:
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Índice:
WPRIM
Assunto principal:
Polissacarídeos
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Soroalbumina Bovina
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Ensaio de Imunoadsorção Enzimática
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Heparina
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Interferon gama
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Metabolismo
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Métodos
Idioma:
Zh
Revista:
Journal of Zhejiang University. Medical sciences
Ano de publicação:
2004
Tipo de documento:
Article