Generation of Dendritic Cells from Human Cord Blood CD34(+) Cells and Their Activation of T Cell-Dependent Antitumor Immune Response In Vitro / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 188-190, 2001.
Article
em Zh
| WPRIM
| ID: wpr-354995
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WPRO
ABSTRACT
To induce the growth and differentiation of dendritic cells (DCs) from human cord blood, CD34(+) cells isolated from human cord blood by mini-MACS were cultured in a liquid culture system with rhSCF, rhGM-CSF, rhTNF-alpha and rhFL for 10 days. Then the induced cells were characterized by DC's morphological and phenotypic properties. In addition, they stimulated the proliferation of allogeneic T cells and possessed an efficient capacity for initiating T cell-dependent antitumor immune responses in vitro. It is concluded that mature DCs could be obtained from human cord blood CD34(+) cells.
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Idioma:
Zh
Revista:
Journal of Experimental Hematology
Ano de publicação:
2001
Tipo de documento:
Article