Study on expression of cell surface L-selectin and soluble L-selectin in patients with acute leukemia / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 251-255, 2003.
Article
em Zh
| WPRIM
| ID: wpr-355671
Biblioteca responsável:
WPRO
ABSTRACT
To study the relationship between the level of the soluble L-selectin (sL-selectin) in plasma and surface L-selectin expression on leukemic cells and episode and state of illness in acute leukemia patients, the plasma level of sL-selectin was measured by a sandwich enzyme-linked immunosorbent assay, and the expressions of surface L-selectin and its gene (lyam-1) were detected by immunohistochemistry and RT-PCR. The results showed that the levels of sL-selectin were significantly higher in untreated and therapy-resistant acute leukemia patients, and expression of L-selectin mRNA and cell surface L-selectinin in untreated and NR patients were significantly lower than that in CR patients and control group (P < 0.05). The plasma levels of sL-selectin were significantly increased in patients with splenomegaly and hepatomegaly (extramedullary infiltration). The levels of sL-selectin were related to the clinical course of the acute leukemia patients. A significant correalation existed between expressions of L-selectin mRNA and surface L-selectin in acute leukemia patients (gamma = 0.782, P < 0.05). It is concluded that expression of L-selectin gene was down-regulated in level of mRNA and protein in acute leukemia patients and both changes were highly correlated. Monitoring of the plasma level of sL-selectin is possibly useful for early diagnosis of relapse and extramedullary infiltration in acute leukemia.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Patologia
/
Solubilidade
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Sangue
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RNA Mensageiro
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Células da Medula Óssea
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Leucócitos Mononucleares
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Leucemia Mieloide Aguda
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Regulação Neoplásica da Expressão Gênica
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Selectina L
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Reação em Cadeia da Polimerase Via Transcriptase Reversa
Tipo de estudo:
Screening_studies
Limite:
Adolescent
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Adult
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Aged
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Child
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Female
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Humans
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Male
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Ano de publicação:
2003
Tipo de documento:
Article