Tissue factor expression in human umbilical vien endothelial cells stimulated by TNF-alpha and its molecular mechanism / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 124-127, 2003.
Article
em Zh
| WPRIM
| ID: wpr-355701
Biblioteca responsável:
WPRO
ABSTRACT
The objective of this study was to explore tissue factor (TF) expression induced by TNF-alpha in cultured human umbilical vien endothelial cells (HUVEC) and its molecular mechanism. TF expression on the surface of HUVEC, TF mRNA and nuclear factor kappaB (NF-kappaB) in HUVEC were detected by flow cytometry, RT-PCR and Western blot respectively. The results showed that TNF-alpha could enhance TF expression on the surface of HUVEC, the TF expression increase was highly consistent with the increased synthesis of TF mRNA, and the increase of TF expression was lately appeared for several hours. It was also found activation of NF kappaB at the time TF mRNA increase. In conclusion, NF-kappaB could be activated promptly after HUVEC incubated with TNF-alpha, then it was bound to TF promotor to start the TF transcription, TF mRNA expression was upregulated, that leaded to the increase of TF expression on the HUVEC surface and activated the coagulation cascade.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Farmacologia
/
Fisiologia
/
Veias Umbilicais
/
RNA Mensageiro
/
Tromboplastina
/
Células Cultivadas
/
Regulação da Expressão Gênica
/
NF-kappa B
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Fator de Necrose Tumoral alfa
/
Células Endoteliais
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Ano de publicação:
2003
Tipo de documento:
Article