Investigation of in vitro hematopoietic differentiation of embryonic stem cell line established from C57BL/6 mice / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 7-10, 2003.
Article
em Zh
| WPRIM
| ID: wpr-355727
Biblioteca responsável:
WPRO
ABSTRACT
Embryonic hematopoiesis in mammals is characterized by successive temporal and spatial changes. Previous investigations indicate that in vitro differentiation of embryonic stem cells (ES cells) derived from 129 mice can mimic embryonic hematopoiesis to some extent. To investigate the in vitro hematopoietic differentiation capacity of ES cells derived from C57BL/6 mice, the authors initially established the murine ES cell line with standard identification methods employed. Next, two-step culture system was utilized for embryoid bodies formation and the appearance of different hematopoietic precursors was confirmed by CFC assay, cellular chemical staining as well as RT-PCR. The results demonstrated that the ES cell line MES-1 fulfilled the criteria of ES cell line and its progeny after in vitro differentiation included primitive and definitive erythrocyte precursors, mixed colony-forming cells and granulocyte/macrophage colony-forming cells. RT-PCR analysis revealed the molecular consistence of transcription factors and hematopoietic markers with cellular event. In conclusion, MES-1 established from C57BL/6 mice was able to differentiate in vitro to a variety of hematopoietic precursors, thus could partly recapitulate embryonic hematopoiesis.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Células-Tronco
/
Fatores de Tempo
/
Fatores de Transcrição
/
Células-Tronco Hematopoéticas
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Células Precursoras Eritroides
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Expressão Gênica
/
Diferenciação Celular
/
Linhagem Celular
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Eritroblastos
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Ensaio de Unidades Formadoras de Colônias
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Ano de publicação:
2003
Tipo de documento:
Article