Your browser doesn't support javascript.
loading
Effects of diazoxide on the mitochondrial ultrastructure and permeability in donor rat myocardium / 中国应用生理学杂志
Article em Zh | WPRIM | ID: wpr-356228
Biblioteca responsável: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of diazoxide (DE) on the myocardial ultrastructure and opening of maitochondrial permeability transition pore (MPTP) in donor rat heart suffered from long-term hypothermic preservation.</p><p><b>METHODS</b>The Langendorff model of isolated rat heart was used. The hearts were stored in 4 degrees C Celsior solution containing different concentration of DE (15, 30, or 45 micromol/L) for 9 h followed by 60 min of reperfusion. The recovery of rate-pressure product (RPP) was observed. The opening of MPTP and myocardial mitochondria ultrastructure were also evaluated.</p><p><b>RESULTS</b>(1) As compared with the celsior solution preserved group, DE (30 micromol/L) increased recovery of RPP during reperfusion and inhibited the opening of MPTP. DE also alleviated the myocardial mitochondrial ultrastucture damage induced by long-term hypothermic preservation. (2) The above effects of DE were attenuated by a mitoK(ATP) channel inhibitor 5-hydroxydecanoate and a MPTP opener atractyloside.</p><p><b>CONCLUSION</b>In the donor rat heart, DE protects myocardial mitochondria ultrastructure against long-term hypothermic preservation injury via inhibiting the opening of MPIP.</p>
Assuntos
Texto completo: 1 Índice: WPRIM Assunto principal: Farmacologia / Fisiologia / Técnicas In Vitro / Canais de Potássio / Distribuição Aleatória / Criopreservação / Ratos Sprague-Dawley / Soluções para Preservação de Órgãos / Proteínas de Transporte da Membrana Mitocondrial / Diazóxido Limite: Animals Idioma: Zh Revista: Chinese Journal of Applied Physiology Ano de publicação: 2010 Tipo de documento: Article
Texto completo: 1 Índice: WPRIM Assunto principal: Farmacologia / Fisiologia / Técnicas In Vitro / Canais de Potássio / Distribuição Aleatória / Criopreservação / Ratos Sprague-Dawley / Soluções para Preservação de Órgãos / Proteínas de Transporte da Membrana Mitocondrial / Diazóxido Limite: Animals Idioma: Zh Revista: Chinese Journal of Applied Physiology Ano de publicação: 2010 Tipo de documento: Article