Development of A synthetic positive control for the nucleic acid detection of mumps virus / 病毒学报
Chinese Journal of Virology
; (6): 544-547, 2013.
Article
em Zh
| WPRIM
| ID: wpr-356669
Biblioteca responsável:
WPRO
ABSTRACT
To rapidly identify the cross-contamination problems caused by the positive control in the process of mumps virus nucleic acid detection, a new mumps virus RNA positive control was developed in this study. Using the same primers and reaction conditions, the cross-contamination problems caused by the positive control could be readily identified by comparing the fragments lengths of the PCR products between the positive control and the samples. This new RNA positive control of mumps virus can be widely used in the diagnosis and genotyping of mumps virus as a better laboratory quality control.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Padrões de Referência
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Virologia
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RNA Viral
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Linhagem Celular
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Primers do DNA
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Reação em Cadeia da Polimerase Via Transcriptase Reversa
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Diagnóstico
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Genética
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Genótipo
/
Métodos
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Virology
Ano de publicação:
2013
Tipo de documento:
Article