Supporting Effect of Umbilical Cord Blood-Derived Mesenchymal Stem Cells on CD34+ Cell Proliferation and Its Mechanism / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 802-808, 2015.
Article
em En
| WPRIM
| ID: wpr-357268
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the ability of UCB-derived MSCs to support the expansion of HSCs ex vivo and the possible mechanisms involved in this process.</p><p><b>METHODS</b>HSCs from UCB were co-cultured with UCB-derived MSCs for 14 days, and then the total number of HSCs and colony-forming units (CFU) were detected. Cytokines levels of MSCs supernatant were analyzed using ELISA.</p><p><b>RESULTS</b>The proliferation rate of HSCs co-cultured with MSCs was significantly higher than that of cultured HSCs alone (P<0.05). Furthermore, the addition of exogenous cytokines to the culture system significantly increased the proliferation rate of HSCs (P<0.05). MSCs had secretion of many cytokines, including GM-CSF, IL-7, IL-8, IL-11, SCF and SDF-1α.</p><p><b>CONCLUSION</b>UCB-derived MSCs as a feeder layer can be an alternative approach for ex vivo expansion of HSCs, and the cytokines by secreted UCB-MSCs may mediate the supportive role of MSC to HSC proliferation.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Citocinas
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Técnicas de Cocultura
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Antígenos CD34
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Proliferação de Células
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Sangue Fetal
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Células-Tronco Mesenquimais
Limite:
Humans
Idioma:
En
Revista:
Journal of Experimental Hematology
Ano de publicação:
2015
Tipo de documento:
Article