Xuefu zhuyu decoction containing serum in vitro induced expressions of desmin and alpha-actin: an experimental research / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
; (12): 1252-1255, 2013.
Article
em Zh
| WPRIM
| ID: wpr-359275
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe whether Xuefu Zhuyu Decoction (XZD) could induce the differentiation of mesenchymal stem cells (MSCs) into cardiac myoid cells, thus seeking for safe and effective inducers.</p><p><b>METHODS</b>The serum pharmacological method was used to induce. XZD containing serum was prepared. MSCs were isolated and cultured. The serum cytotoxicity was detected by MTT. The third generation of favorably grown cells was selected in this experiment. Cells were divided into three groups, i.e., the vehicle control group, the XZD containing serum induced group, and the 5-azacytidine induced group. Expressions of Desmin and alpha-actin were detected by immunocytochemical staining method.</p><p><b>RESULTS</b>Before induction protein expressions of Desmin and alpha-actin were negative, and few was weakly positive. There was no statistical difference in the weak positive expression rate among the 3 groups (P > 0.05). After induction protein expressions of Desmin and alpha-actin were negative, and few was weakly positive in the vehicle control group. Protein expressions of Desmin and alpha-actin were positive in the XZC containing serum induced group and the 5-azacytidine induced group. There was statistical difference in the positive expression rate when compared with the vehicle control group (P > 0.05).</p><p><b>CONCLUSIONS</b>XZD played a role in in vitro inducing differentiation MSCs to cardiac myoid cells. It might participate in expressions of Desmin and alpha-actin.</p>
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WPRIM
Assunto principal:
Farmacologia
/
Células da Medula Óssea
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Medicamentos de Ervas Chinesas
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Células Cultivadas
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Actinas
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Ratos Wistar
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Biologia Celular
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Soro
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Desmina
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Células-Tronco Mesenquimais
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Integrated Traditional and Western Medicine
Ano de publicação:
2013
Tipo de documento:
Article