Preparation of polyclonal antibody against human MxA protein and its specificity to diversified myxovirus resistant protein A / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 76-82, 2010.
Article
em En
| WPRIM
| ID: wpr-360620
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the human myxovirus resistant protein A (MxA), a specifically induced peptide by interferon I, and to use its level as a diagnostic criterion for viral infections.</p><p><b>METHODS</b>Anti-MxA antisera from immunized mice were prepared with the expressed MxA protein of pET32a-MxA in E. coli BL-21(DE3). To confirm the antiserum activity and specificity, the expression product of BL21, wild type MxA pEGFP-C1-wMxA and site-directed mutant MxA pEGFP-C1-mMxA(N589S) stably transfected 3T3 cells and induced A549 cells were detected by Western blot with the antisera using non-MxA transfected or non-IFN-beta induced cells, intact A549, NIH 3T3 cells transfected with pEGFP-C1 and pET32a (+)-transformed BL-21 as controls.</p><p><b>RESULTS</b>The antisera had specific positive immunoreactivity to the NIH3T3 cells transformed with pEGFP-C1-wMxA and pEGFP-C1-mMxA, INF-beta induced A549 cells and BL21 proteins expressed with pET32a (+)-MxA. The hybridization signals from IFN-beta induced A549 cells depended on the IFN-beta inducing concentrations. Meanwhile, immunohistochemical assay showed that NIH 3T3 cells with pEGFP-C1-wMxA and pEGFP-C1-mMxA had > 98% of positive cells at 1:50 dilution of the serum and A549 cells induced by 20 ng/mL IFN-beta for 48 h showed 95% positive cells. pEGFP-C1-transfected NIH 3T3 cells were all negative.</p><p><b>CONCLUSION</b>Anti-sera are highly specific to diversified MxAs. The antibody is detectable by Western blot, immunocytochemistry and immunofluorescence assay.</p>
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Índice:
WPRIM
Assunto principal:
Especificidade da Espécie
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Regulação da Expressão Gênica
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Proteínas de Ligação ao GTP
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Linhagem Celular Tumoral
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Células NIH 3T3
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Alergia e Imunologia
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Proteínas de Resistência a Myxovirus
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Genética
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Metabolismo
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Especificidade de Anticorpos
Limite:
Animals
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Humans
Idioma:
En
Revista:
Biomedical and Environmental Sciences
Ano de publicação:
2010
Tipo de documento:
Article