Relationship between-160C/A polymorphism in CDH1 gene promoter and protein expression in hepatocellular carcinoma / 中华肝胆外科杂志
Chinese Journal of Hepatobiliary Surgery
; (12): 110-112, 2009.
Article
em Zh
| WPRIM
| ID: wpr-396472
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WPRO
ABSTRACT
Objective To investigate the relationship between-160C/A single nucleotide poly-morphism(SNP) in the CDH1 gene promoter and protein expression in hepatocellular carcinoma. Methods Samples were taken from 34 patients with hepatocellular carcinoma and -160 C/A SNP of CDH1 gene promoter was detected by blood specimens adopting direct DNA sequencing. The expres-sion of E-cadherin which encoded by CDH1 gene was determined by paraffin-embedded tissue using immunohistochemistry and the association between -160 C/A SNP and protein expression was ana-lyzed. Results The high and low expression of E-cadherin was observed in 18 cases(52.9%) and 16 cases(47.1%). In these two groups, the difference of the incidence between CC genotype and CA, AA genotype was statistically significant(P<0.05) whereas there was no marked difference between CA and AA genotype. The difference of both A and C allele frequency was significant(P<0.05). Conclusion The -160 C/A SNP of the CDH1 gene promoter may play an important role in regulating the expression of E-cadherin in hepatocellular carcinoma and the incidence of A allele is associated with down-regulation of E-cadherin expression.
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WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Hepatobiliary Surgery
Ano de publicação:
2009
Tipo de documento:
Article