Molecular Cloning and Characterization of a Putative Promoter Region of mPC-1 Gene Homologous to hPC-1 / 中国生物化学与分子生物学报
Chinese Journal of Biochemistry and Molecular Biology
; (12): 856-861, 2006.
Article
em Zh
| WPRIM
| ID: wpr-408580
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ABSTRACT
To identify the regulatory region that are responsible for the expression of mPC-1, we have isolated and characterized the mPC-1 gene promoter. Sequence analysis of the mPC-1 5' -flanking region and a series of truncated constructs were performed, which were transiently transfected into the prostate cancer cell lines and non-prostate cancer cell lines and analyzed through Dual-luciferase reporter assay system. The relative activity of mPC-1 gene promoter was by far higher than pGL3-control containing SV40 promoter and enhancer and p61-PSA containing hPSA 6 kb promoter in AR (androgen receptor, AR ) -positive prostate cancer cell lines. The region from 599 bp to 449 bp of mPC-1 promoter might contain a negative regulatory element. The expression of mPC-1 1.1 kb fragment is mainly restricted into prostate cancer cell lines. The relative activity of mPC-1 1.1 kb 5'-flanking region was regulated by androgen. The results demonstrated that the 1.1 kb fragment of mPC-1 5' -flanking region was relatively strong and prostate cancer cell specific promoter region.The 1.1 kb promoter of mPC-1 gene might be well suited to prostate cancer gene therapy if the promoter was properly modified.
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Índice:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Biochemistry and Molecular Biology
Ano de publicação:
2006
Tipo de documento:
Article