Activation of Macrophage by Polysaccharide Isolated from Salicornia herbacea L / 체질인류학회지
Korean Journal of Physical Anthropology
; : 117-124, 2006.
Article
em Ko
| WPRIM
| ID: wpr-41154
Biblioteca responsável:
WPRO
ABSTRACT
Salicornia herbacea L. is one of the halophytes that can grow in salt marshes, or salt fields along the seashores in Korea. The objective of this study is to investigate the mechanism by which Salicornia Polysaccharide, (SPS) activates macrophages. To analyze macrophage activation and iNOS gene expression, we performed nitrite generation assay, immunohistochemistry, and reverse transcriptase-polymerase chain reaction. A polysaccharide isolated from the Salicornia herbacea L. significantly induces nitric oxide (NO). Immunohistochemical staining of inducible NO synthase (iNOS) showed that the increase of NO was due to the induction of iNOS production. RT-PCR analysis showed that SPS produced significant induction of iNOS gene expression. Immunohistochemical staining of p65 showed that SPS produced strong induction of NF-kappa B/Rel nuclear translocation. Electrophoretic mobility shift assay further confirmed the activation of NF-kappaB/Rel by SPS. In conclusion, we demonstrate that SPS stimulates the macrophages to express iNOS gene via the activation of NF-kappa B/Rel.
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Índice:
WPRIM
Assunto principal:
Imuno-Histoquímica
/
Expressão Gênica
/
Óxido Nítrico Sintase
/
Chenopodiaceae
/
Ensaio de Desvio de Mobilidade Eletroforética
/
Áreas Alagadas
/
Plantas Tolerantes a Sal
/
Coreia (Geográfico)
/
Ativação de Macrófagos
/
Macrófagos
País/Região como assunto:
Asia
Idioma:
Ko
Revista:
Korean Journal of Physical Anthropology
Ano de publicação:
2006
Tipo de documento:
Article