In vitro expansion of human CD8+ CD28- suppressor T cells and their immunological regulatory effect with antigen specificity / 中华器官移植杂志
Chinese Journal of Organ Transplantation
; (12): 614-617, 2011.
Article
em Zh
| WPRIM
| ID: wpr-422486
Biblioteca responsável:
WPRO
ABSTRACT
Objective To study the In vitro expansion of human CD8+ CD28- suppressor T cells and their immunological regulatory effect.Methods Human CD8 + CD28- suppressor T cells were expanded in vitro driven by the combination of cytokines and allogeneic antigen presenting cells (APCs).Flow cytometry was used to assess the development of CD28- subpopulation.Expanded CD8+ CD28- T cells were isolated by immunomagnetic microspheres and then added as third part modulators into mixed lymphocyte culture to assess their immunological regulatory characteristics.Results The combination of cytokines included IL-2,IL-7 and IL-15 and allogeneic APCs could increase the portion of CD8 + CD28- T cell subtype,and expansion fold of CD8+ CD28- T cell subtype was significantly increased as compared with others (P<0.05).Expanded CD8+ CD28- T cells could suppress the proliferation of CD4+ T cells stimulated by allogeneic APCs.Moreover,this suppression had antigen specificity.Conclusion Human CD8 + CD28- suppressor T cells can be in vitro expanded in large amounts driven by the combination of cytokines and allogeneic APCs.Expanded CD8 + CD28- T cells in this study have antigen specific regulatory characteristics.
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Índice:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Organ Transplantation
Ano de publicação:
2011
Tipo de documento:
Article