Comparative study of acute lung injury induced by intraperitoneal injection of PAAF and PPAF / 中华肝胆外科杂志
Chinese Journal of Hepatobiliary Surgery
; (12): 456-458, 2012.
Article
em Zh
| WPRIM
| ID: wpr-426632
Biblioteca responsável:
WPRO
ABSTRACT
Objective To establish a rat model of acute lung injury (ALI) induced by intraperitoneal injection of pancreatitis associated ascitic fluids (PPAF) and perforative peritonitis ascitic fluids (PAAF).A secondary objective is to study the non-specificity of acute lung injury induced by PAAF.Methods Acute necrotizing pancreatitis (ANP) model and perforative peritonitis (PP) model were established in 120 rats,from which the PAAF and PPAF were collected.Forty-eight rats were randomly divided into three groups:normal saline (NS) group,PAAF group,and PPAF group.Within each group,they were randomly sacrificed at 7h and 12h of surgery.The pathological severity of the lung injury,wet/dry ratio,MPO (myeloperoxidase) in lung,and apoptosis rate of pneumocytes were evaluated and analyzed.Results Lung injury,wet/dry ratio,MPO in lungs,and cell apoptosis were significantly higher in the PAAF group and PPAF group than in the NS group (P<0.01).However,there was no significant difference between PAAF group and PPAF group (P>0.05).Conclusions Both PAAF and PPAF can induce acute lung injury in rats by intraperitoneal injection.However,the acute hung injury induced by PAAF has limited specificity.
Texto completo:
1
Índice:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Hepatobiliary Surgery
Ano de publicação:
2012
Tipo de documento:
Article