PCR-based techniques for the detection of minority mutations / 中华检验医学杂志
Chinese Journal of Laboratory Medicine
; (12): 40-45, 2013.
Article
em Zh
| WPRIM
| ID: wpr-431174
Biblioteca responsável:
WPRO
ABSTRACT
With the development of molecular biology and molecular genetics,more attention has been taken to the advanced techniques which can be used to detect minority mutations.In recent years,many techniques such as amplification refractory mutation system,PCR clamping,pyrophosphorolysis-activated polymerization,co-amplification at lower denaturation temperature-PCR and digital PCR have been developed,which will make the hereditary disease diagnosis,cancer detection,noninvasive antenatal diagnosis step into a new stage in the near future.This review presents an overview of the principles,aoolications,advantages and disadvantages of these techniques.
Texto completo:
1
Índice:
WPRIM
Tipo de estudo:
Diagnostic_studies
Idioma:
Zh
Revista:
Chinese Journal of Laboratory Medicine
Ano de publicação:
2013
Tipo de documento:
Article