Effect of Resveratrol on Angiogenesis of Human Umbilical Vein Endothelial Cells With the Possible Mechanisms / 中国循环杂志
Chinese Circulation Journal
; (12): 643-647, 2014.
Article
em Zh
| WPRIM
| ID: wpr-456364
Biblioteca responsável:
WPRO
ABSTRACT
Objective:
To explore the effect of resveratrol (Res) on angiogenesis of human umbilical vein endothelial cells (HUVEC) with the possible mechanisms in vitro.Methods:
The HUVECs were cultured in 6 groups.①Control group, HUVEC were cultured with high glucose in DMEM,②Res group, the cell were cultured with Res at different concentrations,③Res with PI3K blocker LY294002 (Res+Blocker 1) group, ④Blocker 1 group, HUVEC were cultured with LY294002 alone, ⑤Res with eNOS blocker L-NAME (Res +Blocker 2) group and ⑥Blocker 2 group. The effect of Res on HUVEC proliferation was detected by CCK-8 kit, the protein expressions of p-Akt, p-eNOS were examined by Westin blot analysis, nitric oxide (NO) level was measured by nitrate reduction method and the endothelial cell migration was assayed by transwell chamber method.Results:
① Compared with Control group, HUVEC proliferation increased in Res (1, 5μmol/L ) group, P0.05.④Compared with Control group, the cell migration and tubing formation were higher in Res (5μmol/L) group, PConclusion:
Res could up-regulate NO level via activating PI3-K/Akt/eNOS signaling and therefore, improving the proliferation, migration and tubing formation of HUVEC in vitro.
Texto completo:
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Índice:
WPRIM
Idioma:
Zh
Revista:
Chinese Circulation Journal
Ano de publicação:
2014
Tipo de documento:
Article