Investigation of microRNA-373 function and mechanism in HepG2 cell growth suppression induced by 5-fluorouracil / 中华肝胆外科杂志
Chinese Journal of Hepatobiliary Surgery
; (12): 675-678, 2014.
Article
em Zh
| WPRIM
| ID: wpr-457034
Biblioteca responsável:
WPRO
ABSTRACT
Objective To investigate the inhibitory effect of 5-fluorouracil (5-FU) on hepatocellular carcinoma (HCC) cell growth and to elucidate its potential molecular mechanism.Methods Real-time PCR analysis was conducted to determine the expression of miR-373 in HCC tissue specimens and HCC cell lines.The expression of miR-373 was also evaluated in HepG2 cells after 5-FU treatment.Western blot analysis was performed to detect the protein levels of PPP6C,a verified target of miR-373,with transfection of miR-373 mimics or 5-FU treatment.A rescue assay was conducted to investigate the cell growth in HepG2 cells by using CCK-8.Results miR-373 expression was up-regulated in both HCC tissues and cell lines.miR-373 expression depicted about 2.94-fold augment in HepG2 cells as compared to normal liver cells control (P <0.01).5-FU treatment led to a significant decrease of miR-373 levels (approximately 50%,P <0.01,48 h) and resulted in a marked increase of PPP6C protein (approximately 2.1-fold,48 h) in HepG2 cells.The overexpression of miR-373 could prevent the impact of 5-FU treatment on cell growth in HepG2 cells and CCK-8 assay showed that HepG2 cell growth was rescued approximately 81% and 84% at 24 h (P < 0.05) and 48 h (P < 0.01),respectively.Conclusion 5-FU can repress endogenous miR-373 level,which activates the expression of downstream targeted gene PPP6C,thereby exerting an inhibitory effect on HepG2 cells.
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WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Hepatobiliary Surgery
Ano de publicação:
2014
Tipo de documento:
Article