Comparison of transfection efficiency of two kinds of cells by lipofection and electroporation / 重庆医学
Chongqing Medicine
; (36): 4432-4433, 2014.
Article
em Zh
| WPRIM
| ID: wpr-458332
Biblioteca responsável:
WPRO
ABSTRACT
Objective To compare the transfection efficiency between different transfection methods in human HepG2 and SGC7901/ADM cells so as to provide experimental basis for further study .Methods To electrons fect the enhanced GFP plasmid into HepG2 and SGC7901/ADM cells by lipofection and electroporation methods ,respectively .The survival rates and transfection efficiency were analyzed .Results The efficiency of eGFP vector transfected into HepG2 cells by lipofection was (23 .8 ± 2 .1)% , compared with lipofection method ,the efficiency of eGFP plasmid transfected by electroporation was up to (49 .6 ± 2 .5)% ,and the difference was statistically significant(P<0 .05) .The efficiency of SGC7901/ADM cells by lipofection was (25 .4 ± 1 .3)% ,com‐pared with lipofection method ,the efficiency of electroporation was up to(52 .6 ± 2 .1)% ,and the difference was statistically signifi‐cant(P<0 .05) .This study provides reliable test parameters for electransfection of HepG2 and SGC7901/ADM cells .Conclusion The transfection efficiency of large fragment vector is efficiently improved by electroporation .
Texto completo:
1
Índice:
WPRIM
Idioma:
Zh
Revista:
Chongqing Medicine
Ano de publicação:
2014
Tipo de documento:
Article