Determination of propylene glycol content in recombinant human glucagon-like peptide-1(7-36) injection by GC / 国际药学研究杂志
Journal of International Pharmaceutical Research
; (6): 229-231, 2015.
Article
em Zh
| WPRIM
| ID: wpr-464588
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WPRO
ABSTRACT
Objective To establish a method to detect the content of propylene glycol in the recombinant human glucagon-like peptide-1(rhGLP-1)(7-36) injection by gas chromatography(GC). Methods GC was performed using a quartz capillary column (30 m ×320 μm,0.5 μm) with the mobile phase of N2 and inlet temperature of 230℃ at the flow rate of 1ml/min and column temperature of 250 ℃, the injection volume was 1μl. Results The calibration curve was linear within the range of 0.5-1.5 mg/ml, r=0.9991. The average recovery rate was 100.2%; under different chromatographic conditions, the method had good stability, RSD was less than 2.0%. Conclusion This method is simple, accurate and reproducible, and can be applied in the determination of propylene glycol content in rhGLP-1(7-36) injection.
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WPRIM
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Zh
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Journal of International Pharmaceutical Research
Ano de publicação:
2015
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Article