Benzoxazole Derivative B-98 Ameliorates Dextran Sulfate Sodium-induced Acute Murine Colitis and the Change of T Cell Profiles in Acute Murine Colitis Model / 대한소화기학회지
The Korean Journal of Gastroenterology
; : 33-41, 2013.
Article
em Ko
| WPRIM
| ID: wpr-46505
Biblioteca responsável:
WPRO
ABSTRACT
BACKGROUND/AIMS:
The unique role of enzyme 5-lipoxygenase (5-LO) in the production of leukotrienes makes it a therapeutic target for inflammatory bowel disease (IBD). The aim of this study was to evaluate the effects of B-98, a newly synthesized benzoxazole derivatives and a novel 5-LO inhibitor, in a mouse model of IBD induced by dextran sulfate sodium (DSS).METHODS:
C57BL/6 mice were randomly assigned to four groups normal control, DSS colitis (DSS+saline), low dose B-98 (DSS+B-98 20 mg/kg) and high dose B-98 (DSS+B-98 100 mg/kg). B-98 was administered with 3% DSS intraperitoneally. The severity of the colitis was assessed via the disease activity index (DAI), colon length, and histopathologic grading. The production of inflammatory cytokines interleukin (IL)-6 was determined by RT-PCR. Th cells were examined for the proportion of Th1 cell, Th2 cell, Th9 cell, Th17 cell and Treg cell using intracellular cytometry.RESULTS:
The B-98 group showed lower DAI, less shortening of the colon length and lower histopathologic grading compared with the DSS colitis group (p<0.01). The expression of IL-6 in colonic tissue was significantly lower in the B-98 groups than the DSS colitis group (p<0.05). The cellular profiles revealed that the Th1, Th9 and Th17 cells were increased in the DSS colitis group compared to the B-98 group (p<0.05).CONCLUSIONS:
Our results suggest that acute intestinal inflammation is reduced in the group treated with B-98 by Th1, Th9 and Th17 involved cellular immunity.Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Benzoxazóis
/
Índice de Gravidade de Doença
/
Araquidonato 5-Lipoxigenase
/
Linfócitos T
/
Doença Aguda
/
Sulfato de Dextrana
/
Interleucina-6
/
Inibidores de Lipoxigenase
/
Colite
/
Colo
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
Ko
Revista:
The Korean Journal of Gastroenterology
Ano de publicação:
2013
Tipo de documento:
Article