Cell death type of zebrafish lateral line hair cellsinduced by cisplatin / 中国耳鼻咽喉头颈外科
Chinese Archives of Otolaryngology-Head and Neck Surgery
; (12): 585-589, 2016.
Article
em Zh
| WPRIM
| ID: wpr-502899
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WPRO
ABSTRACT
OBJECTIVE To explore cell death type of lateral line hair cellsinduced by cisplatin in zebrafish. METHODS Zebrafish larva were incubated in 1mM cisplatin solution for 6 hrs to induce about 90%lateral line hair cells loss. Time lapse imaging was used to detect the morphology of cisplatin-incubated hair cells in wildtypezebrafish pre-labelled by live dyes Bodipy TR C5-ceramide and Sytogreen 24. TUNEL assay and In situ anti-active Caspase-3 antibody staining were performed to detect nuclei fragmentation and Caspase-3 activity respectively. RESULTS Compared to control group, hair cells condensationand nuclei fragmentation (P<0.05) were detected in cisplatin-incubated group, and active Caspase-3 activity was also observed after cisplatin addition. CONCLUSION Cisplatinmay induced zebrafish lateral line hair cells loss by Caspase-3-dependent apoptotic pathway.
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WPRIM
Idioma:
Zh
Revista:
Chinese Archives of Otolaryngology-Head and Neck Surgery
Ano de publicação:
2016
Tipo de documento:
Article