Effects of Recombinant Chimeric Toxin Dsg3EC_(1-2)PE40 on T and B Lymphocytes from Pemphigus Vulgaris / 中华皮肤科杂志
Chinese Journal of Dermatology
; (12)2003.
Article
em Zh
| WPRIM
| ID: wpr-525632
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WPRO
ABSTRACT
Objective To study the effects of recombinant chimeric toxin Dsg3EC_(1-2)PE40 on T and B cells from PV patients. Methods The recombinant protein Dsg3EC_(1-2)PE40 was expressed on BL21TrxB (DE3) cells, then identified and purified. ELISPOT assay was used to detect and quantitate autoantibody-producing B cells in different concentrations of recombinant chimeric toxin, and MTT assay and ~3H-TdR assay to observe the metabolism and proliferation of T cells from PV patients in vitro. Results The purity of expressed protein Dsg3EC_(1-2)PE40 was up to 80%. The number of anti-Dsg3 antibody-producing B cells in PBMC from PV patients decreased by 40% with treatment of Dsg3EC_(1-2)PE40, which was significantly lower (P
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WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Dermatology
Ano de publicação:
2003
Tipo de documento:
Article