Protective Effect of Polysccharide Sulfate on Sodium Nitroprusside-induced Neurotoxicity in PC12 Cells / 中国药房

ABSTRACT

OBJECTIVE: To investigate the protective effect of polysccharide on sodium nitroprusside-induced neurotoxicity in PC12 cells. METHODS: PC12 cells were cultured and divided into control,damage,PSS(50,100,150 ?g?mL-1) and nimodipine groups. The cell survival was measured by MTT assay. The apoptosis was measured by flow cytometry. The levels of LDH,MDA,SOD,Ca2+i and the caspase-3 activity were determined as well. RESULTS: Compared with the damage group,PSS could significantly reduce cell death rate,lower the efflux of LDH and production of MDA,increase SOD activity,lower the activities of intracellular Ca2+i and caspase-3 activity,and inhibit apoptosis of PC12 cells. CONCLUSION: The remarkable protective effect of PSS on sodium nitroprusside-induced damage of PC12 cells is possibly related to its antagonistic action on intracellular calcium accumulation and anti-peroxidation of lipid.