Effects of Dibutyl Phthalate on the Ovary of Mice / 环境与健康杂志

ABSTRACT

Objective To determine the effects of dibutyl phthalate(DBP) on female mice reproductive system. Methods The healthy Kunming adult female mice with body weight from 22 g to 30 g were randomly divided into 4 groups,one as reagent control group. The other three as treated groups, 12 female mice per group. In the exposure groups,the mice were administrated by peritoneal injection with DBP at the dose of 400?40?4mg/kg body weight,respectively. The mice in control group were administrated with the same amount of vehicle(dimethyl sulfoxide). All the mice were administrated once a day,continuously for 14 days. The estrous cycles were observed during the period of the experimental study. On the fifteenth and twenty-eightieth days,the mice were sacrificed. The organ coefficients of uterus and ovary were determined,and the histopathological changes of the left ovary were observed. Results The estrous cycles in 400 mg/kg body weight dose group and 40 mg/kg body weight dose group were markedly delayed with a significant dose-effect relationship. The organ coefficients of the uterus in the groups exposed to DBP were significantly higher than that of the control group. On the fifteenth days,the number of mature follicles in 400 mg/kg body weight dose group was less than that of the control group,but the number of atretic follicles in 400 mg/kg body weight dose group and 40 mg/kg body weight dose group were more than that of the control group. On the twenty-eightieth days,the number of atretic follicles in 400 mg/kg body weight dose group was more than that of the control group. Conclusion Dibutyl phthalate had adverse effects on the reproduction-endocrine system of adult female mice. DBP might demonstrate an estrogenic activity in vivo.