Protection of Semen Cuscuta extracts from apoptosis PC12 cell of induced by 1-methyl-4-phenylpyridinium / 中成药
Chinese Traditional Patent Medicine
; (12)1992.
Article
em Zh
| WPRIM
| ID: wpr-574760
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WPRO
ABSTRACT
AIM: To establish an apoptotic model induced by 1-methyl-4-phenylpyridinium ion(MPP~+) in PC12 cell and assess the effect of Semen Cuscuta extracts on protecting PC12 cell from apoptosis induced by MPP~+. METHODS: The cell viability was analyzed by MTT method,the morphological changes were observed by Hoechst 33258 staining assay,and the apoptosis rate was measured by flow cytometry analysis(FCM).(RESULTS:) Treatment of 0.15 mM MPP~+ for 48 h induced apoptosis in PC12 cells;concentration of 50 mg/L Semen Cuscuta extracts could protect cells from apoptosis induced by MPP~+. CONCLUSION: MPP~+-induced apoptosis in PC12 cell is greatly inhibited by Semen Cuscuta extracts.
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WPRIM
Idioma:
Zh
Revista:
Chinese Traditional Patent Medicine
Ano de publicação:
1992
Tipo de documento:
Article