Digitized fingerprints of Panax Ginseng rubra by HPLC / 中成药

ABSTRACT

AIM: To establish a HPLC digitized fingerprints of Panax Ginseng rubra to control its quality. METHODS: The chromatographic fingerprints were determined by injecting 20 ?L of the sample solution each time on a Century SIL C_ 18 AQ column (20 cm?4.6 mm, 5 ?m) with the gradient elution solvent system composed of 0.1 mol/L NaH_2PO_4 aqueous solution-acetonitrile. The flow rate was 1 mL/min, the column temperature was maintained at (30?0.15)℃ and the signals were acquired at 203 nm. The chromatographic fingerprints were evaluated by both the chromatographic fingerprint index (F) and the information index of chromatographic fingerprint (I) and so on. RESULTS: 30 co-possessing peaks were selected as the fingerprint peaks and the similarities between each of the ten batches and the referential chromatographic fingerprints of Panax Ginseng rubra were calculated while taking adenosine peak as the reference peak. CONCLUSION: This method with good characteristics and specificity can be used in the quality control of Panax Ginseng rubra.