The study for expression of PEG-3 in rats’ cardiac muscle after electrical injury by the method of immunohistochemistry / 重庆医科大学学报

ABSTRACT

Objective:To study the expression of PEG-3 in cardiac muscle after electrical injury in order to find the time dependablity and the discrimination for the antemortem electrified and postmotorm electrified.MethedsThe animals were devided into two groupsthe negative group and the experimental group.The experimental group were devided into three groups againthe electrical injury group,the electrouction group and the group of electrical shock after death.The electrical injury group is for 0h、3h、6h、12h、24h、3d、6d、12d,the electrouction group is for 0h、1h、3h、6h、12h,and the group of electrical shock after death is for 0h、0.5h、1h、3h,the control group is for 0h、0.5h、1h、3h、6h、12h.After all these we should get the cardiac tissue at the propotional time,and then dye it by the method of HE and immunohistochemistry.Then we can gain the conclusion through the method of semiquantitative analysis.Results:The expression of PEG-3 increased in heart injury early,it may be a useful molecular index to diagnose heart injury induced by electric force.In additional,the rule of PEG-3 expression was changed following time,which may help to estimate the injury interval in forensic EI field.We can also discriminate the the electrouction group and the postmotorm electrified group,because there are only extremely tenuous positive staining for the group of electrical shock after death.Conclusion:PEG-3 can diagnose the electrical injury and discriminate the electrical injury,weather it formed at antemortem or postmortem.