Modulation of T-lymphocyte subsets production with imiquimod in the PBLN cell cultures from ovalbumin-sensitized rats / 中华检验医学杂志
Chinese Journal of Laboratory Medicine
; (12)2003.
Article
em Zh
| WPRIM
| ID: wpr-585085
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WPRO
ABSTRACT
Objective To observe the effect and mechanism of imiquimod on T helper (Th) cell subsets in the Parabronchial lymph node (PBLN) cell cultures from ovalbumin (OVA)-sensitized rats.Methods PBLN were isolated and cultured. PBLN cells were divided into A~F, according to different concentrations of intervention. Cultured for 0, 3, 6, 12, 24, 48 hours, the expressions of IL-4 and IFN-? in supernatants were determined by ELISA. The mRNA expressions of the cytokines in cells were detected by RT-PCR.Results In the group A, only low concentrations of IFN-? were detected. Based on the cultured time, the concentrations of IFN-? were increased significantly if imiquimod concentration was between 1 and 10 ?g/ml. Levels of IL-4 were increased slowly compared with those in the group B (P0.05).Conclusion Imiquimod show the best effect on antigen-specific Th cell subsets when cultured for 12h. The results suggest that imiquimod have benefit in atopic diseases such as the late inflammation reaction of asthma.
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Índice:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Laboratory Medicine
Ano de publicação:
2003
Tipo de documento:
Article