Experimental study on in vitro preparation of adoptive immunotherapy cells induced by CD40 in combination with cytokines / 中国免疫学杂志
Chinese Journal of Immunology
; (12): 933-936,941, 2014.
Article
em Zh
| WPRIM
| ID: wpr-599358
Biblioteca responsável:
WPRO
ABSTRACT
Objective:
To explore a new method of the cultivation of adoptive immunotherapy cells.Methods:
Mononuclear cells was isoplated by density gradient centrifugation and then proliferated by using CD 40-agonist monoclonal antibody 5C11、cytokine of IFN-αand IL-7(CD40 group)in vitro.During the culturing procedure ,the cell morphology was obersved by optical microscope.The percentage of T-lymphocytes, NK-T cells, Treg cells and the cell proliferation, which were compared with CIK group CD3mAb activated,was detected on the 9th day.Results:
There was no significant difference of CD 4+/CD8+T cells percentage between the two groups.But the Treg cells percentage of CIK group was far higher than that of CD 40 group,while the percentage of CD3+CD56+NK-T cells was lower than that of CD 40 group.And a group of Mo-NK-DC cells were observed in the CD 40 group.Conclusion:
The new method of adoptive immunity therapy has been established in this study could increase the percentage of NK -T cells which had the ability to kill tumor cells.Simultaneously ,it is reduced the amount of Treg cells significantly.
Texto completo:
1
Índice:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Immunology
Ano de publicação:
2014
Tipo de documento:
Article