Simultaneous Determination of Bavachin and Bakuchiol in Psorslea corylifolia by HPLC / 中国药房
China Pharmacy
; (12): 3400-3402, 2017.
Article
em Zh
| WPRIM
| ID: wpr-607083
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WPRO
ABSTRACT
OBJECTIVE:To establish a method for content determination of bavachin and bakuchiol in Psoralea corylifolia.METHODS:HPLC method was adopted.The determination was performed on Shim-Pack VP-ODS column with mobile phase consisted of acetonitrile-water (gradient elution) at the flow rate of 1.0 mL/min.The detection wavelength was set at 246 nm,the column temperature was 30 ℃,and the sample size was 10 μL.RESULTS:The linear ranges of bavachin and bakuchiol were 48.9-489 ng(r=0.999 8),784-7 840 ng(r=0.999 9).RSDs of precision,stability and reproducibility tests were all lower than 2.0%.Average recoveries ranged 95.30%-99.63% (RSD=1.45%,n=9),96.89%-100.82% (RSD=1.36%,n=9).CONCLUSIONS:The method is simple,accurate,stable and reproducible,and can be used for the simultaneous determination of bavachin and bakuchiol in P corylifolia.
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WPRIM
Idioma:
Zh
Revista:
China Pharmacy
Ano de publicação:
2017
Tipo de documento:
Article