Function of lncRNA TUG1 in Insulin Secretion from Pancreatic Beta Cells / 现代生物医学进展
Progress in Modern Biomedicine
; (24): 4847-4851, 2017.
Article
em Zh
| WPRIM
| ID: wpr-615055
Biblioteca responsável:
WPRO
ABSTRACT
Objective:
To determine the role of lncRNA TUG1 in pancreatic β cells functioning both in vitro and in vivo.Methods:
The lncRNA TUG1 expression in mice pancreas,brain,muscle and other different tissues was examined through qRT-PCR.MTT,flow cytometry,GSIS,ELISA and immunochemistry analyses were performed to detect the effect of lncRNA TUG1 on insulin secretion in vitro and in vivo.Results:
lncRNA TUG1 was highly expressed in pancreatic tissue compared with other organ tissues.Knockdown of lncRNA TUG1 expression resulted in decreased insulin secretion in β cells both in vitro and in vivo.Immunochemistry analyses showed decreased relative islet area after treatment with lncRNA TUG1 siRNA.Conclusions:
Downregulation of lncRNA TUG1 expression can affect insulin secretion in pancreatic β cells in vitro and in vivo,and lncRNA TUG1 may represent a factor that regulates the function of pancreatic β cells.
Texto completo:
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Índice:
WPRIM
Idioma:
Zh
Revista:
Progress in Modern Biomedicine
Ano de publicação:
2017
Tipo de documento:
Article